| 质粒名称: | pBABE puro H-Ras V12 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 9051 |
| 存储实验室: | William Hahn |
|
载体骨架基本信息 |
|
| 载体名称: | pBabe puro |
| 载体出品公司: | Clontech |
| 空载体大小: | 5169bp |
| 完整质粒大小: | 5812bp |
| 载体类型: | 哺乳细胞表达, Retroviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Hras V12、ev-Ha-ras、Hras |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 700bp |
| 基因突变: | G12V, constitutively active |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed |
| 3’克隆位点: | EcoRI (not destroyed) |
| 5’测序引物 | pBABE-5 |
| 3’测序引物 | pBABE-3 |
|
文献引用方法 |
|
| 材料和方法部分: | pBABE puro H-Ras V12 was a gift from William Hahn (Addgene plasmid # 9051 ; http://n2t.net/addgene:9051 ; RRID:Addgene_9051) |
买家导航
pBABE puro H-Ras V12载体质粒基本信息
pBABE puro H-Ras V12质粒图谱载体图谱和pBABE puro H-Ras V12载体序列质粒序列多克隆位点信息
pBABE puro H-Ras V12质粒载体简介
pBABE puro H-Ras V12质粒序列载体序列
LOCUS pBABE puro H-Ras V12 5812 bp DNA circular SYN 15-DEC-2025
DEFINITION Retroviral expression of a constitutively active form of H-Ras.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5812)
TITLE Viral vectors.
REFERENCE 2 (bases 1 to 5812)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: pBABE puro H-Ras V12
FEATURES Location/Qualifiers
source 1..5812
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 17..486
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 551..750
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 951..1367
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1297..1319
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1335..1351
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS join(1422..1454,1455..1457,1458..1991)
/codon_start=1
/product="human oncoprotein generated by the G12V mutation
in the small GTPase H-Ras"
/label=H-Ras (G12V)
/translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
RQHKLRKLNPPDESGPGCMSCKCVLS"
primer_bind complement(2151..2171)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2156..2485
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2336..2471
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2495..3094
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2495..2514)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2991..3011
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 3315..3784
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(3914..3931)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4085..4673)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4165..4184)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(4833..5624,5625..5693))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5456..5475
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5694..5798)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aagtgccacc tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
61 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
121 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
181 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
241 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
301 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
361 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
421 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
481 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
541 accgggaggc aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga
601 ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc
661 gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact
721 ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt
781 caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
841 ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg
901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg
961 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
1021 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
1081 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
1141 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
1201 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
1261 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
1321 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc cggatcccag
1381 tgtggtggta cagcttgggg ggccagggcc ggggccgagc gatgacggaa tataagctgg
1441 tggtggtggg cgccgtcggt gtgggcaaga gtgcgctgac catccagctg atccagaacc
1501 attttgtgga cgaatacgac cccactatag aggattccta ccggaagcag gtggtcattg
1561 atggggagac gtgcctgttg gacatcctgg ataccgccgg ccaggaggag tacagcgcca
1621 tgcgggacca gtacatgcgc accggggagg gcttcctgtg tgtgtttgcc atcaacaaca
1681 ccaagtcttt tgaggacatc caccagtaca gggagcagat caaacgggtg aaggactcgg
1741 atgacgtgcc catggtgctg gtggggaaca agtgtgacct ggctgcacgc actgtggaat
1801 ctcggcaggc tcaggacctc gcccgaagct acggcatccc ctacatcgag acctcggcca
1861 agacccggca gggagtggag gatgccttct acacgttggt gcgtgagatc cggcagcaca
1921 agctgcggaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
1981 tgctctcctg acgcagcaca agctcaggac atggaggtgc cggatgcagg aaggaggtgc
2041 agacggaagg aggaggaagg aaggacggaa gcaaggaagg aaggaagggc tgctggagcc
2101 cagtcacccc ccaagctgta ggaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg
2161 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2221 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
2281 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
2341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
2401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
2461 ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg
2521 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
2581 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
2641 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
2701 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
2761 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
2821 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
2881 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
2941 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
3001 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
3061 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
3121 gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca
3181 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
3241 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
3301 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
3361 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
3421 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
3481 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
3541 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
3601 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
3661 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
3721 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
3781 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
3841 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
3901 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3961 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
4021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
4081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
4141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4501 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4621 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
4681 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
4741 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc
4801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
4861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
4921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
4981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
5041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
5101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
5161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
5221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
5281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
5341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
5461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
5701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
5761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aa
//
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